Amnis Imaging Flow Cytometry combines these two techniques: Flow Cytometry achieving high event rates flowing objects with single cell discrimination of multiple biomarkers AND High resolution imaging of multiple spectral images and imaging modes.
Similarly, high resolution imaging with high content analysis has created another vastly increasing knowledge base of spatial biology at sub-micron resolution further enhancing our ability to relate biological phenomenon. High color, high throughput flow cytometry has increasingly provided efficient single cell, multi-marker analyses essential to inform biology and medical discovery. “Amnis Imaging Flow Cytometry: High-Content Imaging AND Flow Cytometry from One Platform Creating New Efficiencies and Unique High Value, Single Cell Assays”
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In this seminar, using experimental data as examples when possible, we will discuss some essential best practices, how to avoid some common mistakes, and other important considerations needed to fully utilize the power of flow cytometry. Although we are equipped with better tools than ever before to do flow cytometry, it can still be a complex technique.
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Improvements in software and hardware design have also made flow cytometry more user-friendly and enabled adoption beyond the traditional core of immunological researchers. With the emergence of next-generation fluorochromes, thousands of new antibodies, a diverse array fluorescent proteins, phospho-protein detection, and now assays for RNA expression, flow cytometry is rapidly being adopted by almost every major discipline in the biological sciences. “Taking Your Flow Cytometry to the Next Level – Best Practices and Pitfalls to Avoid”įlow cytometry has become a powerful tool for biological research, capable of generating volumes of valuable data when employed correctly or mountains of misleading data if potential pitfalls aren’t avoided.
Ramiro Diz, PhD, Product Specialist, Flow Cytometry, Thermo Fisher
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